Unai Emery put his players through their final training session on Wednesday at London Colney, before flying out for the last-16 tie.
Other than Alexandre Lacazette who is suspended, the only players missing for the Gunners should be long-term absentees Hector Bellerin, Rob Holding and Danny Welbeck.
Lucas Torreira was sent off at the weekend and will serve a three-match Premier League ban, but is available to feature against Rennes.
Stephan Lichtsteiner, who was not in the matchday squad against Spurs, also trained on Wednesday at London Colney. As did Eddie Nketiah, who will travel to France in the absence of Lacazette.
Petr Cech will start in goal for the Gunners, returning to the club where he spent three years before moving to Chelsea in 2004.
Arsenal squad vs Rennes:
Cech, Leno, Maitland-Niles, Sokratis, Koscielny, Mustafi, Monreal, Kolasinac, Guendouzi, Torreira, Xhaka, Ramsey, Iwobi, Suarez, Mkhitaryan, Ozil, Aubameyang, Nketiah.